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Collagen II Antibody#33340

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Product Detail

Product NameCollagen II Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous levels of total Collagen II protein.

Immunogen TypePeptide

Immunogen DescSynthesized peptide derived from human Collagen II.

Target NameCollagen II

ConjugateUnconjugated

Other Nameschondrocalcin; CO2A1; collagen alpha-1 (II); collagen type II alpha 1;

Accession NoSwiss-Prot: P02458
NCBI Gene ID: 1280

Uniprot P02458

Gene ID 1280;

Sdspage MW140kd

Concentration1.0mg/ml

FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Collagen II antibody #33340.
Immunofluorescence analysis of COS7 cells, using Collagen II antibody #33340.
Western blot analysis of extracts from COLO205 cells, using Collagen II antibody #33340.
Western blot analysis of extracts from 3T3 cells (Lane 2), using Collagen II antiobdy #33340. The lane on the left is treated with synthesized peptide.
Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.

Seema K. Tiwari-Woodruff, J. Cell Biol., Apr 2001; 153: 295.
Alexander Gow, J. Neurosci., Aug 2004; 24: 7051 - 7062.
Anne Florin, Endocrinology, Mar 2005; 146: 1532 - 1540.
Shin-ichiro Kitajiri, J. Cell Sci., Oct 2004; 117: 5087 - 5096.

If you have published an article using product 33340, please notify us so that we can cite your literature.

et al,Composite Hydrogel Containing Collagen-Modified Polylactic Acid-Hydroxylactic Acid Copolymer Microspheres Loaded with Tetramethylpyrazine Promotes Articular Cartilage Repair , (2024),
PMID:
et al,Enhancing auricular reconstruction: A biomimetic scaffold with 3D-printed multiscale porous structure utilizing chondrogenic activity ink. , (2025),
PMID: 39968521

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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