Product Detail
                                
                                                                        Product NameCollagen II Antibody
                                                                                 Host SpeciesRabbit
                                         ClonalityPolyclonal
                                                                                PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
                                        ApplicationsWB IHC IF
                                        Species ReactivityHu Ms Rt
                                        SpecificityThe antibody detects endogenous levels of total Collagen II protein. 
                                        Immunogen TypePeptide
                                         Immunogen DescSynthesized peptide derived from human Collagen II.
                                         Target NameCollagen II 
                                         ConjugateUnconjugated
                                                                                 Other Nameschondrocalcin; CO2A1; collagen alpha-1 (II); collagen type II alpha 1; 
                                        Accession NoSwiss-Prot: P02458
NCBI Gene ID: 1280
                                        Uniprot
                                        P02458
                                        
                                        Gene ID
                                        1280;
                                        
                                                                                Sdspage MW140kd
                                        Concentration1.0mg/ml
                                         FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
                                        StorageStore at -20˚C
                                                                                                                                                         
                                Application Details
                                Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
                             
                            
                                                                
                                    
                                    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Collagen II antibody #33340.
                                 
                                                                
                                    
                                    Immunofluorescence analysis of COS7 cells, using Collagen II antibody #33340.
                                 
                                                                
                                    
                                    Western blot analysis of extracts from COLO205 cells, using Collagen II antibody #33340.
                                 
                                                                
                                    
                                    Western blot analysis of extracts from 3T3 cells (Lane 2), using Collagen II antiobdy #33340. The lane on the left is treated with synthesized peptide.
                                 
                                                             
                            
                                Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.
Seema K. Tiwari-Woodruff, J. Cell Biol., Apr 2001; 153: 295.
Alexander Gow, J. Neurosci., Aug 2004; 24: 7051 - 7062.
Anne Florin, Endocrinology, Mar 2005; 146: 1532 - 1540.
Shin-ichiro Kitajiri, J. Cell Sci., Oct 2004; 117: 5087 - 5096.
                            
                            
                                If you have published an article using product 33340, please notify us so that we can cite your literature.
                                                                                                    
                                      et al,Composite Hydrogel Containing Collagen-Modified Polylactic Acid-Hydroxylactic Acid Copolymer Microspheres Loaded with Tetramethylpyrazine Promotes Articular Cartilage Repair
 ,  (2024),
PMID:
                                     
                                 
                                                                                                                                        
                                      et al,Enhancing auricular reconstruction: A biomimetic scaffold with 3D-printed multiscale porous structure utilizing chondrogenic activity ink.
 ,  (2025),
PMID:
                                    39968521