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Collagen III Antibody#33341

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Product Detail

Product NameCollagen III Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous levels of total collagen III protein.

Immunogen TypePeptide

Immunogen DescSynthesized peptide derived from internal of human collagen III.

Target NameCollagen III

ConjugateUnconjugated

Other NamesCollagen alpha 1; Collagen alpha 1(III) chain [Precursor];

Accession NoSwiss-Prot: P02461
NCBI Gene ID: 1281

Uniprot P02461

Gene ID 1281;

Sdspage MW138kd

Concentration1.0mg/ml

FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500

Immunohistochemistry analysis of paraffin-embedded human pancreas tissue, using Collagen III antibody #33341.
Immunofluorescence analysis of HeLa cells, using Collagen III antibody #33341.
Western blot analysis of extracts from Hela, using Collagen III Antibody.,The lane on the left was treated with the antigen-specific peptide.

Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of GPR56 in the developing brain and binding to GPR56 inhibits neuronal migration and activates the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12.

Arja Lamberg, J. Biol. Chem., May 1996; 271: 11988.
GW Conrad, J. Cell Biol., Mar 1980; 84: 501.
DR Keene, J. Cell Biol., Nov 1987; 105: 2393.
HM Wang, J. Histochem. Cytochem., Nov 1980; 28: 1215.

If you have published an article using product 33341, please notify us so that we can cite your literature.

et al,Enhancing auricular reconstruction: A biomimetic scaffold with 3D-printed multiscale porous structure utilizing chondrogenic activity ink. , (2025),
PMID: 39968521

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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