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Location： Home > Polyclonal Antibodies > GRP78 Antibody

GRP78 Antibody#33395

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Product Detail

Product NameGRP78 Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous levels of total GRP78 protein.

Immunogen TypePeptide

Immunogen DescSynthesized peptide derived from human GRP78.

Target NameGRP78

ConjugateUnconjugated

Other NamesBIP; GLUCOSE-REGULATED PROTEIN 78-KD; HEAT-SHOCK 70-KD PROTEIN 5; IMMUNOGLOBULIN HEAVY CHAIN-BINDING PROTEIN; heat shock 70kDa protein 5 (glucose-regulated protein

Accession NoSwiss-Prot: P11021
NCBI Gene ID: 3309

Uniprot P11021

Gene ID 3309;

Sdspage MW75kd

Concentration1.0mg/ml

FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GRP78 antibody #33395.
Immunofluorescence analysis of COS7 cells, using GRP78 antibody #33395.
Western blot analysis of extracts from HUVEC, HepG2 and Hela cells, using GRP78 antibody #33395.
Western blot analysis of extracts from HuvEc cells (Lane 2), COS7 cells (Lane 3) and JK cells (Lane 4), using GRP78 antiobdy #33395. The lane on the left is treated with systhesized peptide.

Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.

Uma K. Misra, J. Biol. Chem., Oct 2002; 277: 42082 - 42087.
CA Clairmont, J. Biol. Chem., Feb 1992; 267: 3983 - 3990.
J Tooze, J. Cell Biol., Jul 1989; 109: 35.
Jong K. Yun, PNAS, Dec 1997; 94: 13903.

If you have published an article using product 33395, please notify us so that we can cite your literature.

et al,Tauroursodeoxycholic acid mediates endoplasmic reticulum stress and autophagy In adrenocortical carcInoma cells. In Oncol Lett on 2019 Dec by Huang X, Wu L, et al..PMID:31814847 , (2019),
PMID: 31814847
et al,Nerve Growth Factor Protects the Ischemic Heart via Attenuation of the Endoplasmic Reticulum Stress Induced Apoptosis by Activation of Phosphatidylinositol 3-Kinase.In Int J Med Sci on 2015 Jan 1 by Ke Wei, Li Liu et al..PMID: 25552923 , (2015),
PMID: 25552923
et al,Cetuximab enhances oridonin-induced apoptosis through mitochondrial pathway and endoplasmic reticulum stress in laryngeal squamous cell carcinoma cells. In Toxicol In Vitro on 2020 May 12 by Ning Kang, Shijie Cao, et al..PMID:32407876 , (2020),
PMID: 32407876
et al,Endoplasmic reticulum stress is involved in retinal injury induced by repeated transient spikes of intraocular pressure. In J Zhejiang Univ Sci B on 2021 Sept 15 by Xue Yang, Xiaowei Yu et al..PMID: 34514754 , (2021),
PMID: 34514754
et al,Tauroursodeoxycholic acid mediates endoplasmic reticulum stress and autophagy in adrenocortical carcinoma cells , (2019),
PMID: 31814847

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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