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iNOS Antibody#33424

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Product Detail

Product NameiNOS Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous levels of total iNOS protein.

Immunogen TypePeptide

Immunogen DescSynthesized peptide derived from human iNOS.

Target NameiNOS

ConjugateUnconjugated

Other NamesNITRIC OXIDE SYNTHASE; INDUCIBLE; NITRIC OXIDE SYNTHASE; MACROPHAGE; NOS2A

Accession NoSwiss-Prot: P35228
NCBI Gene ID: 4843

Uniprot P35228

Gene ID 4843;

Sdspage MW130kd

Concentration1.0mg/ml

FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using iNOS antibody #33424.
Immunofluorescence analysis of HepG2 cells, using iNOS antibody #33424.
Western blot analysis of extracts from Hela cells, using iNOS antibody #33424.
Western blot analysis of extracts from JK cells (Lane 2), using iNOS antiobdy #33424. The lane on the left is treated with synthesized peptide.

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.

Rachel J. Jones, Am J Physiol Heart Circ Physiol, Jun 2007; 292: H2634 - H2642.
Katrin Linker,Nucleic Acids Res., Aug 2005; 33: 4813 - 4827.
Donna-Marie McCafferty,Donna-Marie McCafferty,
Lian-Qun Qiu, J. Am. Soc. Nephrol., Aug 2004; 15: 2066 - 2078.

If you have published an article using product 33424, please notify us so that we can cite your literature.

et al,The Hippo-YAP signaling pathway drives CD24-mediatedimmune evasion in esophageal squamous cell carcinoma viamacrophage phagocytosis. In Oncogene on 2024 Feb by Xiaofeng Zhou, Ziyi Yan,et al..PMID:38168654 , (2024),
PMID: 38168654
et al,Identification of Cytochrome P450 2E1 as a Novel Target in Neuroinflammation and Development of Its Inhibitor Q11 as a Treatment Strategy. , (2025),
PMID: 40122152

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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