Product Detail
Product NameHMGB1 Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous levels of total HMGB1 protein.
Immunogen TypePeptide
Immunogen DescSynthesized peptide derived from internal of human HMGB1.
Target NameHMGB1
ConjugateUnconjugated
Other NamesHMG-1; HMG1; High mobility group protein 1; SBP-1; amphoterin
Accession NoSwiss-Prot: P09429
NCBI Gene ID: 3146
Uniprot
P09429
Gene ID
3146;
Sdspage MW25kd
Concentration1.0mg/ml
FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
Western blotting: 1:500~1:3000
Western blot analysis of extracts from Jurkat cells, HepG2 cells, COLO205 cells and 293 cells, using HMGB1 antibody #33661.
DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells By similarity.
Wen L., Nucleic Acids Res. 17:1197-1214(1989).
Ferrari S., Genomics 35:367-371(1996).
Rasmussen R.K., Electrophoresis 18:588-598(1997).
If you have published an article using product 33661, please notify us so that we can cite your literature.
et al,Biodegradable iridium coordinated nanodrugs potentiate photodynamic therapy and immunotherapy of lung cancer.
, (2025),
PMID:
39488900
Yes
