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LAMA5 Antibody#34275

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Product Detail

Product NameLAMA5 Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB;IHC;IF

Species ReactivityHu Ms

SpecificityThe antibody detects endogenous levels of LAMA5 protein.

Immunogen TypePeptide

Immunogen DescThe antiserum was produced against synthesized peptide derived from human LAMA5.

Target NameLAMA5

ConjugateUnconjugated

Other Names Laminin subunit alpha-5; KIAA0533; KIAA1907;

Accession NoSwiss-Prot: O15230
NCBI Gene ID: 3911

Uniprot O15230

Gene ID 3911;

Sdspage MW400kd

Concentration1.0mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20˚C

Application Details

WB 1:500 - 1:2000. IHC: 1:100-300 IF 1:100-300 

Western Blot analysis of RAW HepG2 A549 cells
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using LAMA5 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.

This gene encodes one of the vertebrate laminin alpha chains. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. The protein encoded by this gene is the alpha-5 subunit of of laminin-10 (laminin-511), laminin-11 (laminin-521) and laminin-15 (laminin-523)

If you have published an article using product 34275, please notify us so that we can cite your literature.

et al,Ser252Trp mutation in fibroblast growth factor receptor 2 promotes branching morphogenesis in mouse salivary glands. In J Oral Biosci on 2024 Mar by Daiki Iwata, Kaori Kometani-Gunjigake,et al..PMID:38246420 , (2024),
PMID: 38246420

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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