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RPL35 Antibody#34357

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Product Detail

Product NameRPL35 Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous levels of total RPL35 protein.

Immunogen TypePeptide

Immunogen DescSynthesized peptide derived from internal of human RPL35.

Target NameRPL35

ConjugateUnconjugated

Other Names 60S ribosomal protein L35; ribosomal protein L35; RL35;

Accession NoSwiss-Prot: P42766
NCBI Gene ID: 11224

Uniprot P42766

Gene ID 11224;

Sdspage MW18kd

Concentration1.0mg/ml

FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
Western blotting: 1:500~1:3000

Western blot analysis of extracts from HeLa cells, Jurkat cells, COLO cells and 293 cells, using RPL35 antibody #34357.
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L29P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Patel S.K., Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases
Ebert L., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Humphray S.J., Nature 429:369-374(2004).

If you have published an article using product 34357, please notify us so that we can cite your literature.

et al,The cardiac translational landscape reveals that micropeptides are new players involved in cardiomyocyte hypertrophy , (2021),
PMID: 33677093

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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