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BLM Antibody#35370

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Product Detail

Product NameBLM Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were purified by antigen-affinity chromatography.

ApplicationsIF WB

Species ReactivityHu

SpecificityThe antibody detects endogenous levels of total BLM protein.

Immunogen TypeRecombinant Protein

Immunogen DescRecombinant fragment corresponding to a region within amino acids 30 and 385 of BLM.

Target NameBLM

ConjugateUnconjugated

Other NamesBS antibody; MGC126616 antibody; MGC131618 antibody; MGC131620 antibody; RECQ2 antibody; RECQL2 antibody; RECQL3 antibody; BLM antibody; "DNA helicase; RecQ-like type 2 antibody"; Bloom syndrome protein antibody; recQ protein-like 3 antibody; "Bloom syndr

Accession NoSwiss-Prot#:P54132;
NCBI Gene#:641

Uniprot P54132

Gene ID 641;

Sdspage MW159kd

Concentration0.93mg/ml

FormulationRabbit IgG in 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

StorageStore at -20˚C

Application Details
Western blotting: 1:500-1:3000
Immunofluorescence: 1:100-1:1000
BLM antibody detects BLM protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
5 % SDS-PAGE
#35370 diluted at 1:500
BLM antibody, N-term detects BLM protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BLM protein stained by BLM antibody, N-term (#35370) diluted at 1:500.
Blue: Hoechst 33342 staining.
The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination. [provided by RefSeq]

If you have published an article using product 35370, please notify us so that we can cite your literature.

et al,Specific Monitoring the DNA Helicase Function via Anchor-Embedded DNA Probe , (2024),
PMID:

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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