Product Detail
Product NameHA Tag Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntigen affinity purification.
ApplicationsWB
SpecificityThe antibody detects transfected proteins contanining HA tag.
Immunogen TypePeptide
Immunogen DescSynthetic peptide: YPYDVPDYA (Human influenza hemagglutinin-HA-epitope) conjugated to KLH.
Target NameHA Tag
ConjugateUnconjugated
Other NamesHA epitope tag; Hemagglutinin Tag
Sdspage MW55kd
Concentration0.5mg/ml
FormulationRabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
StorageStore at -20˚C
Application Details
Western blotting: 1:500-1:5000
Gel: 8%SDS-PAGE
Lysate: 2ug Recombinant HA tagged protein
Primary antibody: 1/500 dilution
Secondary antibody dilution: 1/8000
Exposure time: 1 second
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
If you have published an article using product 35534, please notify us so that we can cite your literature.
et al,Phosphoglycerate Kinase 1 Phosphorylates Beclin1 to Induce Autophagy.In Mol Cell on 2017 Mar 2 by Xu Qian , Xinjian Li,et al..PMID: 28238651
, (2017),
PMID:
28238651
et al,PTEN Suppresses Glycolysis by Dephosphorylating and Inhibiting Autophosphorylated PGK1
, (2019),
PMID:
31492635
Yes
