Product Detail
Product NameFLAG tag Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntigen affinity purification.
ApplicationsWB Elisa
SpecificityThe antibody detects transfected proteins contanining FLAG tag.
Immunogen TypePeptide
Immunogen DescSynthetic peptide: 3*DYKDDDDK conjugated to KLH
Target NameFLAG tag
ConjugateUnconjugated
Other NamesDDDDK epitope tag; DYKDDDDK epitope tag
Sdspage MW50/40 kDa
Concentration0.8mg/ml
FormulationRabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
StorageStore at -20˚C
Application Details
WB 1:10000-1:50000 ELISA:1:5000-1:10000
The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. Due to its small size, it is unlikely to affect the tagged protein's biochemical properties. This antibody recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins. (The background information is obtained from official databases and does not represent the product information.)
If you have published an article using product 35535, please notify us so that we can cite your literature.
et al,PKC蔚 phosphorylates MIIP and promotes colorectal cancer metastasis through inhibition of RelA deacetylation.
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et al,GlcNAcylation of fumarase maintains tumour growth under glucose deficiency.
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et al,MLL2 promotes cancer cell lymph node metastasis by interacting with RelA and facilitating STC1 transcription.In Cell Signal on 2020 Jan; by Li H, Li Q, et al..PMID:31676369
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et al,O-GlcNAcylation of PFKFB3 is required for tumor cell proliferation under hypoxia.In Oncogenesis on 2020 Feb 14 by Lei Y, Chen T, et al..PMID:32060258
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et al,Upregulation of ZNF148 in SDHB-deficient gastrointestinal stromal tumor potentiates Forkhead box M1-mediated transcription and promotes tumor cell invasion.
In Cancer Sci on 2020 Apr by Gao X, Ma C, et al..PMID: 32060966
, (2020),
PMID:
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et al,O-GlcNAcylation of Fumarase Maintains Tumour Growth Under Glucose Deficiency .In Nat Cell Biol ON 2017 Jul by Ting Wang , Qiujing Yu,et al..PMID: 28628081
, (2017),
PMID:
28628081
More
et al,PKC蔚 phosphorylates MIIP and promotes colorectal cancer metastasis through inhibition of RelA deacetylation.In Nat Commun on 2017 Oct 16 by Tao Chen, Jingjie Li ,et al..PMID: 29038521
, (2017),
PMID: 29038521
et al,Interaction between NS1 and Cellular MAVS Contributes to NS1 Mitochondria Targeting. In Viruses on 2021 Sep 23 by Yeu-Yang Tseng, Chih-Ying Kuan,et al..PMID:34696339
, (2021),
PMID: 34696339
Yes
