Product Detail
Product Namec-Myc Tag Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntigen affinity purification.
ApplicationsWB
SpecificityThe antibody detects transfected proteins contanining c-Myc Tag.
Immunogen TypePeptide
Immunogen DescSynthetic peptide: C-EQKLISEEDL conjugated with KLH.
Target Namec-Myc Tag
ConjugateUnconjugated
Other Namesc- Myc epitope Tag
Accession NoSwiss-Prot:P01106
Gene ID:4609
Uniprot
P01106
Gene ID
4609;
Sdspage MW55kd
Concentration1mg/ml
FormulationRabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
StorageStore at -20˚C
Application Details
Western blotting: 1:1000-1:5000
Gel: 10%SDS-PAGE
Lysate:2ug Recombinant HA tagged protein
Primary antibody: 1/1000 dilution
Secondary antibody dilution: 1/8000
Exposure time: 1 second
A c-myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A c-myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting. It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
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