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ACTIN Antibody#35537

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Product Detail

Product NameACTIN Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntigen affinity purification.

ApplicationsWB,ELISA

Species ReactivityPlants

SpecificityThe antibody detects endogenous levels of total ACTIN protein.

Immunogen TypePeptide

Immunogen DescSynthetic peptide of Arabidopsis thaliana actin

Target NameACTIN

ConjugateUnconjugated

Other NamesACTB

Accession NoSwiss-Prot#: P53497
NCBI Gene ID: 823805
Gene Accssion: NP_190236.1

Uniprot P53497

Gene ID 823805;

Calculated MW42kDa

Concentration0.7mg/ml

FormulationRabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

StorageStore at -20˚C

Application Details

Western blotting: 1:500-1:2000

Gel: 8%SDS-PAGELysate: 40 μgLane 1-2: Corn leaves lysate, Rice leaves lysatePrimary antibody: ACTIN Antibody at dilution 1/1000Secondary antibody: Goat anti rabbit IgG at 1/5000 dilutionExposure time: 5 minutes

Member of actin subclass composed of ACT12 and ACT4. RNA is expressed at very low levels in vegetative organs, low levels in flowers and very high levels in pollen. Expression of an ACT12/GUS fusion was found in vascular tissues, tapetum, developing and mature pollen, the root cap and in a ring of pericycle tissues during lateral root initiation and early development.

If you have published an article using product 35537, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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