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TUBB3 Antibody#35539

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Product Detail

Product NameTUBB3 Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntigen affinity purification.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous levels of total TUBB3 protein.

Immunogen TypeRecombinant Protein

Immunogen DescFusion protein corresponding to a region derived from internal residues of human Tubulin beta-3 chain

Target NameTUBB3

ConjugateUnconjugated

Other NamesTUBB4

Accession NoSwiss-Prot#: Q13509
NCBI Gene ID: 10381
Gene Accssion: BC000748

Uniprot Q13509

Gene ID 10381;

Sdspage MW50kd

Concentration0.5mg/ml

FormulationRabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

StorageStore at -20˚C

Application Details
Western blotting: 1:500-1:1000
Immunohistochemistry: 1:25-1:50
Gel: 8%SDS-PAGE
Lysate:30ug Human brain glioma tissue
Primary antibody: 1/500 dilution
Secondary antibody dilution: 1/8000
Exposure time: 10 seconds
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue using #35539 at dilution 1/25.
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #35539 at dilution 1/25.
his gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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