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DDAH1 Antibody#37368

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Product Detail

Product NameDDAH1 Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntigen affinity purification.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous levels of total DDAH1 protein.

Immunogen TypePeptide

Immunogen DescSynthetic peptide corresponding to a region derived from internal residues of human dimethylarginine dimethylaminohydrolase 1

Target NameDDAH1

ConjugateUnconjugated

Other NamesDDAH; HEL-S-16

Accession NoSwiss-Prot#: O94760
NCBI Gene ID: 23576
Gene Accssion: NP_036269/O94760

Uniprot O94760

Gene ID 23576;

Sdspage MW31kd

Concentration3.1mg/ml

FormulationRabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

StorageStore at -20˚C

Application Details
Western blotting: 1:200-1:1000
Immunohistochemistry: 1:50-1:200
Gel: 10%SDS-PAGE
Lysates (from left to right): Human fetal liver tissue
Amount of lysate: 40ug per lane
Primary antibody: 1/400 dilution
Secondary antibody dilution: 1/8000
Exposure time: 30 seconds
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #37368 at dilution 1/50.
This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.

If you have published an article using product 37368, please notify us so that we can cite your literature.

et al,DDAH1 Protects against Acetaminophen-Induced Liver Hepatoxicity in Mice . In Antioxidants (Basel) on 2022 Apr 29 by Xiyue Shen, Saddam Muhammad Ishaq, et al..PMID:35624743 , (2022),
PMID: 35624743
et al,DDAH1 Protects against Cardiotoxin-Induced Muscle Injury and Regeneration In Antioxidants (Basel)On2023 Sep 13byFei Feng , Bingqing Cui et al..PMID:37760057 , (2023),
PMID: 37760057
et al,Aerobic Exercise Protects against Cardiotoxin-Induced Skeletal Muscle Injury in a DDAH1-Dependent Manner. In Antioxidants (Basel) on 2024 Sep 1 by Fei Feng, Kai Luo, et al..PMID:39334728 , (2024),
PMID: 39334728

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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