Product Detail
Product NamePIK3R3 Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntigen affinity purification.
ApplicationsWB IHC
Species ReactivityHu Ms
SpecificityThe antibody detects endogenous levels of total PIK3R3 protein.
Immunogen TypePeptide
Immunogen DescSynthetic peptide corresponding to a region derived from internal residues of human phosphoinositide-3-kinase, regulatory subunit 3 (gamma)
Target NamePIK3R3
ConjugateUnconjugated
Other Namesp55; p55-GAMMA
Accession NoSwiss-Prot#: Q92569
NCBI Gene ID: 8503
Gene Accssion: NP_001107644
Uniprot
Q92569
Gene ID
8503;
Sdspage MW54kd
Concentration1mg/ml
FormulationRabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
StorageStore at -20˚C
Application Details
Western blotting: 1:500-1:2000
Immunohistochemistry: 1:25-1:100
Gel: 10%SDS-PAGE
Lysates (from left to right): Mouse brain, human fetal brain and mouse testis tissue, hela cell and human fetal kidney tissue
Amount of lysate: 40ug per lane
Primary antibody: 1/250 dilution
Secondary antibody dilution: 1/8000
Exposure time: 2 minutes
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using #37373 at dilution 1/25.
Phosphatidylinositol 3-kinase is a lipid kinase that phosphorylates the inositol ring of phosphatidylinositol and related compounds at the 3¡¯ position. PI 3-kinase p55 (PIK3R3) is comprised of a catalytic subunit and a regulatory subunit. The human p55 protein is composed of a rare amino terminal region followed by a proline-rich motif and two Src homology 2 (SH2) domains. PI 3-kinase p55 mRNAs are expressed in most human fetal and adult tissues; predominant expression is observed in the adult testis. Splice variant(s) of PI 3-kinase p55 have been identified; one of which has a deletion of 36 amino acids at the amino terminus and another which has an insertion of 59 amino acids at position 256 between the SH2 domains. Research suggests that PI 3-kinase p55 interacts with the IGFIR (Insulin-like growth factor-I receptor) and IR (Insulin receptor) and may be involved in PI 3-kinase activation by these receptors.
If you have published an article using product 37373, please notify us so that we can cite your literature.
et al,CSF-1R regulates non-small cell lung cancer cells dissemination through Wnt3a signaling.
, (2017),
PMID:
29218239
Yes
