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STK3/STK4 Antibody#37462

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Product Detail

Product NameSTK3/STK4 Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntigen affinity purification.

ApplicationsWB IHC

Species ReactivityHu Ms

SpecificityThe antibody detects endogenous levels of total STK3/STK4 protein.

Immunogen TypePeptide

Immunogen DescSynthetic peptide corresponding to a region derived from internal residues of human serine threonine kinase 3/4

Target NameSTK3-STK4

ConjugateUnconjugated

Other NamesKRS1; MST2/KRS2; MST1; YSK3; TIIAC

Accession NoSwiss-Prot#: Q13043
NCBI Gene ID: 6789
Gene Accssion: NP_006273

Uniprot Q13043

Gene ID 6789;

Sdspage MW56kd

Concentration3mg/ml

FormulationRabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

StorageStore at -20˚C

Application Details
Western blotting: 1:500-1:2000
Immunohistochemistry: 1:50-1:200
Gel: 6%SDS-PAGE
Lysates (from left to right): Human bladder carcinoma tissue and A172 cell, human brain malignant glioma tissue
Amount of lysate: 40ug per lane
Primary antibody: 1/1000 dilution
Secondary antibody dilution: 1/8000
Exposure time: 10 seconds
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using #37462 at dilution 1/70.
Sterile-20 (Ste20) is a serine/threonine kinase in Saccharomyces cerevisiae that is involved in relaying signals from G protein-coupled receptors to cyto-solic MAP kinase cascades. Mammalian protein kinases that display sequence similarity to Ste20 are divided into two groups, the PAK subfamily and the GCK subfamily.?The mammalian Ste20-like kinases (MST kinases), also known as Krs proteins, are members of the GCK subfamily. Ksr-1 (MST-2) and Ksr-2 (MST-1) are both direct substrates of caspase-3 that accelerate caspase-3 activation.

If you have published an article using product 37462, please notify us so that we can cite your literature.

et al,RAS-association domain family 1A regulates the abnormal cell proliferation in psoriasis via inhibition of Yes-associated protein , (2021),
PMID: 33960627

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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