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Location： Home > Mouse Monoclonal Antibodies > EGFR Mouse Monoclonal Antibody

EGFR Mouse Monoclonal Antibody#37996

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Product Detail

Product NameEGFR Mouse Monoclonal Antibody

Host SpeciesMouse

ClonalityMonoclonal

PurificationAffinity purification using immunogen.

ApplicationsWB,IHC,IF

Species ReactivityHu

SpecificityThe EGFR Mouse Monoclonal antibody detects endogenous EGFR proteins.

Target NameEGFR

ConjugateUnconjugated

Other NamesReceptor tyrosine-protein kinase ErbB-1; ERBB1

Accession NoSwiss-Prot#:P00533

Uniprot P00533

Gene ID 1956;

Sdspage MW170kd

Concentration1.0mg/ml

FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details

WB dilution: 1:1000
IF dilution:1:100-200
IHC 1:200-1000

Western blot analysis of Hela cell lysate, using #37996 diluted at 1:1,000.
IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades.

If you have published an article using product 37996, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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