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ErbB-2/HER-2 Mouse Monoclonal Antibody#37997

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Product Detail

Product NameErbB-2/HER-2 Mouse Monoclonal Antibody

Clone No.11H9

Host SpeciesMouse

ClonalityMonoclonal

PurificationAffinity purification using immunogen.

ApplicationsWB,IHC,IF

Species ReactivityHu Ms Rt

SpecificityThe ErbB-2/HER-2 Mouse Monoclonal antibody detects endogenous ErbB-2/HER-2 proteins.

Target NameErbB-2/HER-2

ConjugateUnconjugated

Other Namesc-erb B2/neu protein; CD340; erbB-2; ERBB2; HER-2; HER-2/neu; HER2; herstatin; Metastatic lymph node gene 19 protein; MLN 19;

Accession NoSwiss-Prot#:P04626

Uniprot P04626

Gene ID 2064;

Sdspage MW138kd

Concentration1.0mg/ml

FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
WB dilution: 1:2000~1:4000
IHC dilution: 1:200

IF dilution:1:200
Western blot analysis of Hela cell lysate (lane 1) and mouse brain (lane 2), using #37997 diluted at 1:4,000.
IHC staining of human breast cancer tissue with ErbB-2/HER-2 mouse mAb(11H9) diluted at 1:200.
Immunofluorescence analysis of Mouse-spleen tissue. 1,HER2 Monoclonal Antibody(11H9)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Rat-spleen tissue. 1,HER2 Monoclonal Antibody(11H9)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,HER2 Monoclonal Antibody(11H9) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,HER2 Monoclonal Antibody(11H9) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,HER2 Monoclonal Antibody(11H9) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
ErbB 2 is a receptor tyrosine kinase of the ErbB 2 family. It is closely related instructure to the epidermal growth factor receptor. ErbB 2 oncoprotein is detectable in a proportion of breast and other adenocarconomas, as well as transitional cell carcinomas. In the case of breast cancer, expression determined by immunohistochemistry has been shown to be associated with poor prognosis.

If you have published an article using product 37997, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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