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Location： Home > Mouse Monoclonal Antibodies > AMACR/P504S Mouse Monoclonal Antibody

AMACR/P504S Mouse Monoclonal Antibody#37999

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Product Detail

Product NameAMACR/P504S Mouse Monoclonal Antibody

Clone No.4A12

Host SpeciesMouse

ClonalityMonoclonal

PurificationAffinity purification using immunogen.

ApplicationsWB,IHC,IF

Species ReactivityHu

SpecificityAMACR/P504S Mouse Monoclonal antibody detects endogenous AMCAR/P504S proteins.

Target NameAMACR/P504S

ConjugateUnconjugated

Other Names2-methylacyl-CoA racemase; Alpha-methylacyl-CoA racemase; AMACR; CBAS4; RACE

Accession NoSwiss-Prot#:Q9UHK6

Uniprot Q9UHK6

Gene ID 23600;

Sdspage MW42kd

Concentration1.0mg/ml

FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
WB dilution: 1:1000~1:2000
IHC dilution: 1:200

IF dilution:1:200
Western blot analysis of HepG2 cell lysate (lane 1) and mouse kidney (lane 2), using #37999 diluted at 1:1,000.
IHC staining of mouse prostate adenocarcinoma tissue with AMACR/P504S mouse mAb(4A12) diluted at 1:200.
Immunofluorescence analysis of Mouse-kidney tissue. 1,AMACR Monoclonal Antibody(4A12)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Rat-brain tissue. 1,AMACR Monoclonal Antibody(4A12)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1,AMACR Monoclonal Antibody(4A12) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,AMACR Monoclonal Antibody(4A12) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,AMACR Monoclonal Antibody(4A12) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
AMACR has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of AMACR protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia.

If you have published an article using product 37999, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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