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Location： Home > Mouse Monoclonal Antibodies > NFκB p65 Mouse Monoclonal Antibody

NFκB p65 Mouse Monoclonal Antibody#38008

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Product Detail

Product NameNFκB p65 Mouse Monoclonal Antibody

Clone No.14H2

Host SpeciesMouse

ClonalityMonoclonal

PurificationAffinity purification using immunogen.

ApplicationsWB,IHC

Species ReactivityHu Ms Rt

SpecificityNFκB p65 Mouse monoclonal antibody detects endogenous p65 proteins.

Target NameNFκB p65

ConjugateUnconjugated

Other Namesp65; NFKB3

Accession NoSwiss-Prot#:Q04206

Uniprot Q04206

Gene ID 5970;

Sdspage MW65kd

Concentration1.0mg/ml

FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
Western blotting: 1:1000~1:3000
Immunohistochemistry: 1:200
Western blot analysis of Hela (lane 1), 293T(lane 2) and HepG (lane 3), using #38008 diluted at 1:2,000.
Western blot analysis of Mouse Heart (lane 1), Rat Heart (lane 2), Rat Liver (lane 3) and Rat Spleen (lane 4), using #38008 diluted at 1:3,000.
IHC staining of mouse hippocampus tissue with NFκB p65 mouse mAb(14H2) diluted at 1:200.
NFkB p65 is ubiquitinated leading to its proteosomal degradation, which is required for termination of the NFkB response. Phosphorylation of NFkB p65 on S536 stimulates acetylation of K310 by CBP, enhancing transcriptional activity. NFkB p65 is also acetylated at K122, enhancing DNA binding and impairing the interaction with NFKBIA. The protein is deacetylated by HDAC3. Invasion of a host by a pathogen is frequently associated with the activation of NF-kB, which coordinates various aspects of immune function required for resistance to infection.

If you have published an article using product 38008, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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