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Location： Home > Mouse Monoclonal Antibodies > CK7 Mouse Monoclonal Antibody

CK7 Mouse Monoclonal Antibody#38009

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Product Detail

Product NameCK7 Mouse Monoclonal Antibody

Clone No.12D7

Host SpeciesMouse

ClonalityMonoclonal

PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

ApplicationsWB,IHC,IF

Species ReactivityHuman;Mouse

SpecificityThe CK7 Mouse Monoclonal antibody detects endogenous CK7 proteins.

Immunogen DescSynthetic Peptide of CK7

Target NameCK7

ConjugateUnconjugated

Other NamesCK-7; CK7; cytokeratin 7; Cytokeratin-7; K2C7; K7; keratin 7

Accession NoSwiss-Prot#:P08729

Uniprot P08729

Gene ID 3855;

Sdspage MW54kd

Concentration1.0mg/ml

FormulationPBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

StorageStore at -20˚C/1 year

Application Details

WB dilution: 1:1000~1:2000
IHC dilution: 1:200
IF dilution:1:50-200

Western blot analysis of Hela (lane 1), Mouse Kidney (lane 2) and Mouse Brain (lane 3), using #38009 diluted at 1:2,000.
IHC staining of human lung cancer tissue with CK7 mouse mAb(12D7) diluted at 1:200.
Immunofluorescence analysis of human-liver tissue. 1,CK7 Monoclonal Antibody(12D7)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Hela cell.

Cytokeratin 7 is a 54kD intermediate filament protein found in a variety of glandularepithelia. Cytokeratin 7 has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. It is present in the transitional epithelium of bladder as well as ovarian and lungepithelia, and occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed. However, Cytokeratin 7 is not expressed by epithelial cells of the gastrointestinal tract, colon or prostate. Keratin 7 is often co expressed with keratin 19.

If you have published an article using product 38009, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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