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Location： Home > Mouse Monoclonal Antibodies > Carcinoembryonic Antigen(CEA) Mouse Monoclonal Antibody

Carcinoembryonic Antigen(CEA) Mouse Monoclonal Antibody#38016

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Product NameCarcinoembryonic Antigen(CEA) Mouse Monoclonal Antibody

Clone No.1.00E+02

Host SpeciesMouse

ClonalityMonoclonal

PurificationAffinity purification using immunogen.

ApplicationsIHC,IF

Species ReactivityHu

SpecificityThe EFHD1 Rabbit Polyclonal antibody detects endogenous EFHD1 proteins.

Target NameCarcinoembryonic Antigen(CEA)

ConjugateUnconjugated

Other Namescarcinoembryonic antigen SG8; CEA; pregnancy specific beta-1-glycoprotein 2

Accession NoSwiss-Prot#:P11465

Uniprot P11465

Gene ID 5670;

Concentration1.0mg/ml

FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
IHC dilution: 1:200

IF dilution:1:50-200
IHC staining of human colon cancer tissue with CEA mouse mAb(10E1) diluted at 1:200.
Immunofluorescence analysis of Human-lung-cancer tissue. 1,Carcinoembryonic Antigen Monoclonal Antibody(10E1)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,Carcinoembryonic Antigen Monoclonal Antibody(10E1) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
CEA is synthesised during development in the fetal gut, and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. Antibodies to CEA are useful in identifying the origin of various metastatic adenocarcinomas and in distinguishing pulmonary adenocarcinomas (60 to 70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+).

If you have published an article using product 38016, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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