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Mouse Monoclonal Antibodies > Aquaporin 4 Mouse Monoclonal Antibody
Aquaporin 4 Mouse Monoclonal Antibody#38017
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All lanes : Aquaporin 4 Mouse mAb at 1/2k dilution Lane 1 : Hela whole cell lysatesLane 2 : Mouse Heart tissueLane 3 : Rat Heart Tissue All lanes : Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution Predicted band size: 26 kDa Observed band size: 45 kDa Exposure time: 7 seconds
IF analysis of Hela with #38017(Left) and DAPI (Right) diluted at 1:100.
Immunofluorescence analysis of Human-appendix tissue. 1,Aquaporin 4 Monoclonal Antibody(4H1)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Mouse-brain tissue. 1,Aquaporin 4 Monoclonal Antibody(4H1)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,Aquaporin 4 Monoclonal Antibody(4H1) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
NOTE
Application
- WBWestern Blotting
- IHCImmunohistochemistry
- IFImmunofluorescence
- ICCImmunocytochemistry
- FCFlow Cytometry
- IPImmunoprecipitation
- EELISA
- DBDot Blotting
- ChIPChromatin Immunoprecipitation
- GICAGold Immunochromatography Assay
- NCNegative Control
Species Reactivity
- HuHuman
- MsMouse
- RtRat
- DmDrosophila melanogaster
- CCaenorhabditis elegans
- MkMonkey
- RbRabbit
- BBovine
- DDog
- PPig
- HmHamster
- ChHmChinese Hamster
- ChkChicken
- ShpSheep
