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Location： Home > Mouse Monoclonal Antibodies > Ki 67 Mouse Monoclonal Antibody

Ki 67 Mouse Monoclonal Antibody#38019

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Product Detail

Product NameKi 67 Mouse Monoclonal Antibody

Clone No.4A8

Host SpeciesMouse

ClonalityMonoclonal

PurificationAffinity purification using immunogen.

ApplicationsIHC,IF

Species ReactivityHuman

SpecificityThe Ki 67 Mouse Monoclonal antibody detects endogenous Ki 67 protein.s

Target NameKi 67

ConjugateUnconjugated

Other Namesantigen identified by monoclonal antibody Ki-67; Antigen KI-67; KI67; KIA; MKI67

Accession NoSwiss-Prot#:P46013

Uniprot P46013

Gene ID 4288;

Concentration1.0mg/ml

FormulationPBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

StorageStore at -20˚C

Application Details

IHC dilution: 1:200

IF dilution:1:50-200

IHC staining of Mouse Kidney tissue with Ki 67 mouse mAb(4A8) diluted at 1:200.
Immunofluorescence analysis of Human-breast-cancer tissue. 1,Ki 67 Monoclonal Antibody(4A8)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Mouse-testis tissue. 1,Ki 67 Monoclonal Antibody(4A8)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Rat-brain tissue. 1,Ki 67 Monoclonal Antibody(4A8)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,Ki 67 Monoclonal Antibody(4A8) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,Ki 67 Monoclonal Antibody(4A8) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis. The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. It is strictly associated with cell proliferation. During interphase, the Ki-67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. Ki-67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0).

If you have published an article using product 38019, please notify us so that we can cite your literature.

et al,Glycogen Phosphorylase B Is Regulated by miR101-3p and Promotes Hepatocellular Carcinoma Tumorigenesis. In Front Cell Dev Biol on 2020 Nov 25 by Guangying Cui, Huifen Wang, et al..PMID:33324633 , (2020),
PMID: 33324633
et al,Circular RNA circPPM1F modulates M1 macrophage activation and pancreatic islet inflammation in type 1 diabetes mellitus. In Theranostics on 2020 Aug 29 by Caiyan Zhang, Xiao Han, et al..PMID:33042261 , (2020),
PMID: 33042261
et al,NAP1L1 interacts with hepatoma-derived growth factor to recruit c-Jun inducing breast cancer growth. In Cancer Cell Int on 2021 Nov 13 by Shu Liu, Yewei Zhang, et al..PMID:34774047 , (2021),
PMID: 34774047
et al,Supplementing a specific synbiotic suppressed the incidence of AOM/DSS-induced colorectal cancer in mice In iScienceOn2023 May 28byHuixia Wu , Zhengchun Wu et al..PMID:37378327 , (2023),
PMID: 37378327
et al,Application of MRI-Based Radiomics in Preoperative Prediction of NF2 Alteration in Intracranial Meningiomas , (2022),
PMID: 36267986

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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