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Location： Home > Mouse Monoclonal Antibodies > MAP2 Mouse Monoclonal Antibody

MAP2 Mouse Monoclonal Antibody#38022

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Product Detail

Product NameMAP2 Mouse Monoclonal Antibody

Clone No.7D4

Host SpeciesMouse

ClonalityMonoclonal

PurificationAffinity purification using immunogen.

ApplicationsIHC,IF

Species ReactivityHu Ms Rt

SpecificityThe MAP2 Mouse Monoclonal antibody detects endogenous MAP2 protein.s

Target NameMAP2

ConjugateUnconjugated

Other NamesDKFZp686I2148; MAP-2; MAP2; MAP2A; MAP2B; MAP2C; Microtubule-associated protein 2

Accession NoSwiss-Prot#:P11137

Uniprot P11137

Gene ID 4133;

Concentration1.0mg/ml

FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
IHC dilution: 1:200

IF dilution:1:50-200
IHC staining of paraffin-embedded Human brain tissue with MAP2 mouse mAb£¨7D4£©diluted at 1:200.
Immunofluorescence analysis of Mouse-brain tissue. 1,MAP2 Monoclonal Antibody(7D4)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Rat-brain tissue. 1,MAP2 Monoclonal Antibody(7D4)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,MAP2 Monoclonal Antibody(7D4) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,MAP2 Monoclonal Antibody(7D4) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1,MAP2 Monoclonal Antibody(7D4) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present, while MAP2a is absent. Between postnatal days 10 and 20, MAP2a appears. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and when neurons have reached their mature morphology. MAP2 is degraded by a Cathepsin D-like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in other types. MAP2 is known to promote microtubule assembly and to form side-arms on microtubules. It also interacts with neurofilaments, actin, and other elements of the cytoskeleton.

If you have published an article using product 38022, please notify us so that we can cite your literature.

et al,The inhibition of PGAM5 suppresses seizures in a kainate-induced epilepsy model via mitophagy reduction.In Front Mol Neurosci on 2022 Dec 22 by Fuxin Zhong, Yunhao Gan, et al..PMID:36618822 , (2022),
PMID: 36618822

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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