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Fumarase Mouse Monoclonal Antibody(7F1)#38030

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Product NameFumarase Mouse Monoclonal Antibody(7F1)

Clone No.7F1

Host SpeciesMouse

ClonalityMonoclonal

PurificationAffinity purification using immunogen.

ApplicationsWB,IHC,IF

Species ReactivityHu Rt Ms

SpecificityFH Mouse Monoclonal antibody detects endogenous FH proteins.

Target NameFumarase

ConjugateUnconjugated

Other Names FH; Fumarase; fumarate hydratase; Fumarate hydratase, mitochondrial; FUMH; HLRCC; LRCC; MCL; MCUL1

Accession NoSwiss-Prot#:P07954

Uniprot P07954

Gene ID 2271;

Sdspage MW54kd

Concentration1.0mg/ml

FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
WB dilution: 1:2000~1:5000
IHC dilution:1:50-300
IF dilution:1:200
Western blot analysis of 1) 293T, 2) HepG2, 3) Hela cells, using #38030 diluted at 1:3,000.
Western blot analysis of 1) Mouse Brain tissue, 2) Rat Brain tissue,, using #38030 diluted at 1:3,000.
Immunofluorescence analysis of Human-liver-cancer tissue. 1,FH Monoclonal Antibody(7F1)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Mouse-testis tissue. 1,FH Monoclonal Antibody(7F1)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,FH Monoclonal Antibody(7F1) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,FH Monoclonal Antibody(7F1) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,FH Monoclonal Antibody(7F1) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Fumarase (FH) is an enzyme that catalyzes the reversible hydration/dehydration of fumarate to malate. Fumarase comes in two forms: mitochondrial and cytosolic. The mitochondrial isoenzyme is involved in the Krebs Cycle (also known as the Tricarboxylic Acid Cycle [TCA] or the Citric Acid Cycle), and the cytosolic isoenzyme is involved in the metabolism of amino acids and fumarate. Subcellular localization is established by the presence of a signal sequence on the amino terminus in the mitochondrial form, while subcellular localization in the cytosolic form is established by the absence of the signal sequence found in the mitochondrial variety.

If you have published an article using product 38030, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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