Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > Mouse Monoclonal Antibodies > Carbonic Anhydrase IX Mouse Monoclonal Antibody

Carbonic Anhydrase IX Mouse Monoclonal Antibody#38033

Datasheet PDF
Availability： Yes
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(0) Other
Product Detail

Product NameCarbonic Anhydrase IX Mouse Monoclonal Antibody

Host SpeciesMouse

ClonalityMonoclonal

PurificationAffinity purification using immunogen.

ApplicationsWB,IHC,IF

Species ReactivityHu

SpecificityCA IX Mouse Monoclonal antibody detects endogenous CA IX protein.s

Target NameCarbonic Anhydrase IX

ConjugateUnconjugated

Other NamesCA-IX; CA9; CAH9; CAIX; Car9; Carbonate dehydratase IX; Carbonic anhydrase 9

Accession NoSwiss-Prot#:Q16790

Uniprot Q16790

Gene ID 768;

Sdspage MW49kd

Concentration1.0mg/ml

FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
WB dilution: 1:3000~1:5000
IP dilution: 1:200
IHC dilution:1:50-300
IF dilution:1:200
Western blot analysis of 1) Hela, 2) 293T, using #38033 diluted at 1:5,000.
1¡¢Input: Hela Cell Lysate
2¡¢IP product: IP dilute 1:200
Western blot analysis: primary antibody : #38033 1:2,000
Secondary antibody: Goat anti-Mouse IgG, Light chain specific, 1:5,000
Immunofluorescence analysis of human-liver-cancer tissue. 1,CA IX Monoclonal Antibody(12F10)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1,CA IX Monoclonal Antibody(12F10) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
The carbonic anhydrases (or carbonate dehydratases) form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs rather slowly in the absence of a catalyst. CAIX is considered to be one of the best cellular biomarkers of hypoxia. Furthermore, recent studies examining the association between CAIX levels and various clinicopathological outcomes suggest that CAIX expression may also be a valuable prognostic indicator for overall survival. Antibodies against CAIX serve as excellent excellent biomarkers of hypoxic regions in many solid tumors

If you have published an article using product 38033, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors