Product Detail
Product NameCK17 Mouse Monoclonal Antibody
Host SpeciesMouse
ClonalityMonoclonal
PurificationAffinity purification using immunogen.
ApplicationsWB,IHC,IF
Species ReactivityHu
SpecificityCK17 Mouse Monoclonal antibody detects CK17 endogenous proteins.
Target NameCK17
ConjugateUnconjugated
Sdspage MW48kd
Concentration1.0mg/ml
FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
WB dilution: 1:1000~1:2000
IP dilution: 1:200
IHC dilution:1:50-300
IF dilution:1:200
Western blot analysis of 1) Hela, 2) MCF7, 3) 293T, using #38038 diluted at 1:2,000.
1¡¢Input: Hela Cell Lysate
2¡¢IP product: IP dilute 1:200
Western blot analysis: primary antibody : #38038 1:1,000
Secondary antibody: Goat anti-Mouse IgG, Light chain specific, 1:5,000
Immunofluorescence analysis of Human-breast tissue. 1,CK17 Monoclonal Antibody(10A1)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,CK17 Monoclonal Antibody(10A1) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Cytokeratin17 (CK17) is a protein that in humans is encoded by the KRT17 gene. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. Mutations in the gene encoding this protein lead to Jackson-Lawler type pachyonychia congenita and steatocystoma multiplex.
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