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Oct1 Mouse Monoclonal Antibody (27-7G1)#38046

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Product Detail

Product NameOct1 Mouse Monoclonal Antibody (27-7G1)

Clone No.27-7G1

Host SpeciesMouse

ClonalityMonoclonal

PurificationAffinity purification using immunogen.

ApplicationsWB,IHC,IF

Species ReactivityHu

SpecificityAntibody detects endogenous Oct1 protein.

Target Name1-Oct

ConjugateUnconjugated

Other NamesNF-A1; Oct-1; OCT1; Octamer-binding protein 1; Octamer-binding transcription factor 1

Accession NoSwiss-Prot#:P14859

Uniprot P14859

Gene ID 5451;

Sdspage MW90kd

Concentration1.0mg/ml

FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details

WB dilution: 1:500~1:2000


IHC dilution:1:50-300
IF dilution:1:200
Western blot analysis of 1) Hela, 2) Jurkat, 3) HepG2, using #38046
Immunofluorescence analysis of Human-breast-cancer tissue. 1,Oct1 Monoclonal Antibody(7G1)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1,Oct1 Monoclonal Antibody(7G1) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

POU domain, class 2, transcription factor 1 is a protein that in humans is encoded by the POU2F1 gene.Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins.

If you have published an article using product 38046, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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