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CD16 Mouse Monoclonal Antibody (Q32)#38047

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Product Detail

Product NameCD16 Mouse Monoclonal Antibody (Q32)

Clone No.Q32

Host SpeciesMouse

ClonalityMonoclonal

PurificationAffinity purification using immunogen.

ApplicationsWB,IHC

Species ReactivityHu

SpecificityAntibody detects endogenous CD16 protein.

Target NameCD16

ConjugateUnconjugated

Other NamesCD32; CD32A; CDw32; Fc fragment of IgG, low affinity IIa, receptor (CD32); Fc-gamma RII-a; Fc-gamma-RIIa; FCG2; FCG2A; FcGR; FCGR2; FCGR2A; FCGR2A1; FcRII-a; IGFR2

Accession NoSwiss-Prot#:P12318

Uniprot P12318

Gene ID 2212;

Sdspage MW35kd

Concentration1.0mg/ml

FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
WB dilution: 1:1000~1:2000
IHC dilution:1:50-300
Western blot analysis of 1) Jurkat, 2)K562 CD16 Mouse mAb(Q32) #38047
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,CD16 Monoclonal Antibody(Q32) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
CD16 is a low affinity Fc receptor.It is a cluster of differentiation found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages.

If you have published an article using product 38047, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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