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Cytokeratin 18 Mouse Monoclonal Antibody (1B3)#38048

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Product NameCytokeratin 18 Mouse Monoclonal Antibody (1B3)

Clone No.1B3

Host SpeciesMouse

ClonalityMonoclonal

PurificationAffinity purification using immunogen.

ApplicationsWB,IF

Species ReactivityHu Rt Ms

SpecificityAntibody detects endogenous Cytokeratin 18 protein.

Target NameCytokeratin 18

ConjugateUnconjugated

Other NamesCK-18; CYK18; cytokeratin 18; Cytokeratin-18; K18; K1C18; keratin 18; Keratin, type I cytoskeletal 18; Keratin-18; KRT18

Accession NoSwiss-Prot#:P05783

Uniprot P05783

Gene ID 3875;

Sdspage MW48kd

Concentration1.0mg/ml

FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
WB dilution: 1:1000~1:3000

IF dilution:1:200
Western blot analysis of 1) HepG2, 2) Hela, 3) Mouse Liver tissue, 4) C2C12, 5) Rat Heart tissue, 6) Mouse Skeletal Muscle tissue, using #38048 diluted at 1:2000.
IF analysis of Hela, and DAPI (Right) diluted at 1:100.
Keratin 18 is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.Keratin 18 is often used together with keratin 8 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood.

If you have published an article using product 38048, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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