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Location： Home > Mouse Monoclonal Antibodies > NFκB p65 Mouse Monoclonal Antibody

NFκB p65 Mouse Monoclonal Antibody#38054

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Product Detail

Product NameNFκB p65 Mouse Monoclonal Antibody

Host SpeciesMouse

ClonalityMonoclonal

PurificationAffinity purification using immunogen.

ApplicationsWB,IHC,IF

Species ReactivityHu Rt Ms

SpecificityNFκB p65 Mouse monoclonal antibody detects endogenous p65 proteins.

Target NameNFκB p65

ConjugateUnconjugated

Other NamesMGC131774; NFKB3; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; p65; RELA; TF65; Transcription factor p65

Accession NoSwiss-Prot#:Q04206

Uniprot Q04206

Gene ID 5970;

Sdspage MW65kd

Concentration1.0mg/ml

FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
WB dilution: 1:1000~1:3000
IF dilution: 1:100
IP dilution: 1:200
IHC dilution:1:50-300
IF dilution:1:200
Western blot analysis of 1) Hela, 2) Rat Heart Tissue, 3) Mouse Spleen Tissue, using #38054 diluted at 1:2000.
IF analysis of Hela with #38054(Left) and DAPI (Right) diluted at 1:100.
1¡¢Input: Hela Cell Lysate
2¡¢IP product: IP dilute 1:200
Western blot analysis: primary antibody :#38054 1:2,000
Secondary antibody: Goat anti-Mouse IgG, Light chain specific, 1:5,000
Immunofluorescence analysis of Human-appendix tissue. 1,NFkB p65 Monoclonal Antibody(5G6)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue. 1,NFkB p65 Monoclonal Antibody(5G6) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,NFkB p65 Monoclonal Antibody(5G6) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
NFkB p65 is ubiquitinated leading to its proteosomal degradation, which is required for termination of the NFkB response. Phosphorylation of NFkB p65 on S536 stimulates acetylation of K310 by CBP, enhancing transcriptional activity. NFkB p65 is also acetylated at K122, enhancing DNA binding and impairing the interaction with NFKBIA. The protein is deacetylated by HDAC3. Invasion of a host by a pathogen is frequently associated with the activation of NF-kB, which coordinates various aspects of immune function required for resistance to infection.

If you have published an article using product 38054, please notify us so that we can cite your literature.

et al,The antioxidant effects of garlic saponins protect PC12 cells from hypoxia-induced damage. In Br J Nutr on 2011 Apr by Hong Luo, Jian Huang, et al..PMID: 21205417 , (2011),
PMID: 21205417
et al,Astragalus polysaccharides mediated preventive effects on bronchopulmonary dysplasia in rats.In Pediatr Res on 2014 Oct by Xiao-Hong Wang, Hong-Ling Jia et al..PMID: 25029259 , (2014),
PMID: 25029259
et al,Costunolide improved dextran sulfate sodium-induced acute ulcerative colitis in mice through NF-kB, STAT1/3, and Akt signaling pathways. In Int Immunopharmacol on 2020 Jul by Fan Xie, Hai Zhang, et al..PMID:32413737 , (2020),
PMID: 32413737
et al,Ischemic postconditioning protects against acute kidney injury after limb ischemia reperfusion by regulating HMGB1 release and autophagyIn Ren FailOn2023 DecbyZhongdi Liu , Yifan Chen et al..PMID:37158301 , (2023),
PMID: 37158301
et al,Research on the role and mechanism of Aloe vera (L.) Burm.f. in the treatment of burn: Based on network pharmacology analysis and experimental verification , (2024),
PMID:

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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