Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > Mouse Monoclonal Antibodies > eIF4A1 Mouse Monoclonal Antibody

eIF4A1 Mouse Monoclonal Antibody#38072

Datasheet PDF
Availability： Yes
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(0) Other
Product Detail

Product NameeIF4A1 Mouse Monoclonal Antibody

Host SpeciesMouse

ClonalityMonoclonal

PurificationAffinity purification using immunogen.

ApplicationsWB,IHC,IF

Species ReactivityHu Rt Ms

SpecificityAntibody detects endogenous eIF4A1 protein.

Target NameeIF4A1

ConjugateUnconjugated

Other NamesATP-dependent RNA helicase eIF4A-1; DDX2A; EIF-4A; eIF-4A-I; EIF4A; eIF4A-I; EIF4A1; Eukaryotic initiation factor 4A-I

Accession NoSwiss-Prot#:P60842

Uniprot P60842

Gene ID 1973;

Sdspage MW48kd

Concentration1.0mg/ml

FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
WB dilution: 1:1000~1:3000
IHC dilution:1:50-300
IF dilution:1:100-200
Western blot analysis of 1) 293T ,2) Hela, 3) HepG2, 4) Mouse Brain tissue, 5) Rat Brain tissue, using #38072 diluted at 1:2,000.
Immunofluorescence analysis of Mouse-spleen tissue. 1,eIF4A1 Monoclonal Antibody(M8)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.
Immunohistochemical analysis of paraffin-embedded Human-colon-cancer tissue. 1,eIF4A1 Monoclonal Antibody(M8) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,eIF4A1 Monoclonal Antibody(M8) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 1,eIF4A1 Monoclonal Antibody(M8) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
The eukaryotic initiation factor-4A (eIF4A) family consists of 3 closely related proteins EIF4A1, EIF4A2, and EIF4A3. These factors are required for the binding of mRNA to 40S ribosomal subunits. Eukaryotic initiation factor (eIF) complex 2 forms a ternary complex with GTP and the initiator Met-tRNA ¨C this process is regulated by guanine nucleotide exchange and phosphorylation and serves as the main regulatory element of the bottleneck of protein expression.

If you have published an article using product 38072, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors