Product Detail
Product Nameβ-actin Mouse Monoclonal Antibody
Clone No.5B7
Host SpeciesMouse
ClonalityMonoclonal
PurificationAffinity purification using immunogen.
ApplicationsWB,IHC
Species ReactivityHu Rt Ms Mk Dg Chk Hm Rb Pg Sh
SpecificityAntibody detects endogenous β-actin protein.
Target Nameβ-actin
ConjugateUnconjugated
Other NamesACTB; BRWS1; PS1TP5BP1
Accession NoSwiss-Prot#:P60709
Uniprot
P60709
Gene ID
60;
Sdspage MW45kd
Concentration1.0mg/ml
FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
Western blotting: 1:5000~1:10000
Immunohistochemistry: 1:200
Western blot analysis of 1) 293T ,2) Mouse Kidney tissue, 3) Hela, 4) Rat Heart tissue, 5) Rat Brain tissue, using #38074 diluted at 1:5,000.
IHC staining of Human ovary tissue with β-Actin mouse mAb(5B7) diluted at 1:200.
β-actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against β-actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-actin may not be stable in certain cells. For example, expression of β-actin in adipose tissue is very low and therefore β-actin should not be used as loading control for these tissues.
If you have published an article using product 38074, please notify us so that we can cite your literature.
et al,Gut-Flora-Dependent Metabolite Trimethylamine-N-Oxide Promotes Atherosclerosis-Associated Inflammation Responses by Indirect ROS Stimulation and Signaling Involving AMPK and SIRT1. In Nutrients on 2022 Aug 15 by Sa Zhou, Jiamin Xue, et al..PMID:36014845
, (2022),
PMID:
36014845
et al,Novel oxicam nonsteroidal compound XK01 attenuates inflammation by suppressing the NF-κB and MAPK pathway in RAW264.7 macrophages. In Heliyon on 2024 Jan 9 by Jixiang Wang, Jiawang Tan,et al..PMID:38312593
, (2024),
PMID:
38312593
Yes
