Product Detail
Product Nameβ-tubulin Mouse Monoclonal Antibody
Clone No.5G3
Host SpeciesMouse
ClonalityMonoclonal
PurificationAffinity purification using immunogen.
ApplicationsWB,IHC
Species ReactivityHu Rt Ms Mk Dg Chk Hm Rb Sh
SpecificityAntibody detects endogenous β-tubulin protein.
Target Nameβ-tubulin
ConjugateUnconjugated
Other Namesbeta 5-tubulin; beta Ib tubulin; beta-4 tubulin; M40; MGC117247; MGC16435; OK/SW-cl.56; TBB5; TUBB; TUBB1; TUBB5
Accession NoSwiss-Prot#:P07437
Uniprot
P07437
Gene ID
203068;
Sdspage MW55kd
Concentration1.0mg/ml
FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
Western blotting: 1:5000~1:10000
Immunohistochemistry: 1:200
Western blot analysis of 1) Hela ,2) Mouse Kidney tissue, 3) Rat Heart tissue, using #38075 diluted at 1:5,000.
IHC staining of Human colon tissue with β-tubulin mouse mAb(5G3) diluted at 1:200.
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.
If you have published an article using product 38075, please notify us so that we can cite your literature.
et al,The effect of exposure time and concentration of airborne PM2.5 on lung Injury In mice: A transcriptome analysis. In Redox Biol on 2019 Sep by Wang H, Shen X et al..PMID:31279222
, (2019),
PMID:
31279222
Yes
