Product Detail
Product NameCleaved PARP Mouse Monoclonal Antibody
Host SpeciesMouse
ClonalityMonoclonal
PurificationAffinity purification using immunogen.
ApplicationsWB,IHC,IF
Species ReactivityHu
SpecificityAntibody detects endogenous pro and active PARP protein.
Target NameCleaved PARP
ConjugateUnconjugated
Other NamesApopain; CASP-3; CASP3; caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; Caspase-3; Caspase-3 subunit p12
Accession NoSwiss-Prot#:P42574
Uniprot
P42574
Gene ID
836;
Sdspage MW89kd
Concentration1.0mg/ml
FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
WB dilution: 1:2000~1:5000
IHC dilution:1:50-300
IF dilution:1:50-200
Western blot analysis of Jurkat, using #38076 diluted at 1:3,000.
Poly [ADP-ribose] polymerase 1 (PARP-1) also known as NAD+ ADP-ribosyltransferase 1 or poly[ADP-ribose] synthase 1 is an enzyme that in humans is encoded by the PARP1 gene. PARP1 has a role in repair of single-stranded DNA (ssDNA) breaks. Knocking down intracellular PARP1 levels with siRNA or inhibiting PARP1 activity with small molecules reduces repair of ssDNA breaks. In the absence of PARP1, when these breaks are encountered during DNA replication, the replication fork stalls, and double-strand DNA (dsDNA) breaks accumulate.
If you have published an article using product 38076, please notify us so that we can cite your literature.
Yes
