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Mouse Monoclonal Antibodies > α Skeletal Muslce Actin Mouse Monoclonal Antibody(4B11)
α Skeletal Muslce Actin Mouse Monoclonal Antibody(4B11)#38090
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Western blot analysis of 1) Hela, 2) Mouse Brain tissue, 3) Rat Brain tissue, using #38090 diluted at 1:20,000.
1¡¢Input: Mouse Brain Tissue Lysate
2¡¢IP product: IP dilute 1:200
Western blot analysis: primary antibody: #38090 1:10,000
Secondary antibody: Goat anti-Mouse IgG, Light chain specific, 1:5,000
2¡¢IP product: IP dilute 1:200
Western blot analysis: primary antibody: #38090 1:10,000
Secondary antibody: Goat anti-Mouse IgG, Light chain specific, 1:5,000
Immunofluorescence analysis of Human-liver-cancer tissue. 1, skeletal muscle actin Monoclonal Antibody(4B11)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Mouse-liver tissue. 1, skeletal muscle actin Monoclonal Antibody(4B11)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Rat-liver tissue. 1, skeletal muscle actin Monoclonal Antibody(4B11)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1, skeletal muscle actin Monoclonal Antibody(4B11) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, skeletal muscle actin Monoclonal Antibody(4B11) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
NOTE
Application
- WBWestern Blotting
- IHCImmunohistochemistry
- IFImmunofluorescence
- ICCImmunocytochemistry
- FCFlow Cytometry
- IPImmunoprecipitation
- EELISA
- DBDot Blotting
- ChIPChromatin Immunoprecipitation
- GICAGold Immunochromatography Assay
- NCNegative Control
Species Reactivity
- HuHuman
- MsMouse
- RtRat
- DmDrosophila melanogaster
- CCaenorhabditis elegans
- MkMonkey
- RbRabbit
- BBovine
- DDog
- PPig
- HmHamster
- ChHmChinese Hamster
- ChkChicken
- ShpSheep
