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β II tubulin Mouse Monoclonal Antibody (Rodent Preferred)#38092

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Product Nameβ II tubulin Mouse Monoclonal Antibody (Rodent Preferred)

Host SpeciesMouse

ClonalityMonoclonal

PurificationAffinity purification using immunogen.

ApplicationsWB,IHC

Species ReactivityRt Ms

SpecificityThe β II tubulin Mouse Monoclonal Antibody detects endogenous β II tubulin protein.

Target Nameβ II tubulin

ConjugateUnconjugated

Accession NoSwiss-Prot:Q13885
Gene ID:7280

Uniprot Q13885

Gene ID 7280;

Sdspage MW50kd

Concentration1.0mg/ml

FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
WB dilution: 1:50000~1:100000
IHC dilution:1:50-300
IP dilution: 1:200
Western blot analysis of 1) Mouse Brain tissue, 2) Rat Brain tissue, using #38092 diluted at 1:100,000.
1¡¢Input: Mouse Brain Tissue Lysate
2¡¢IP product: IP dilute 1:200
Western blot analysis: primary antibody : #38092 1:10,000
Secondary antibody: Goat anti-Mouse IgG, Light chain specific, 1:5,000
Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1, II tubulin Monoclonal Antibody(Mix) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, II tubulin Monoclonal Antibody(Mix) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1, II tubulin Monoclonal Antibody(Mix) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Tubulin is the major constituent of microtubules. It exists as a heterodimer consiting of an alpha and a beta subunit. Class II beta tubulin is the major form of tubulin beta in neurons, although it is not neuronal specific. It is found in many other tissues including lung tissue and Schwann cells.

If you have published an article using product 38092, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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