Product Detail
Product NameDDB1 antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were purified by affinity purification using immunogen.
ApplicationsWB,IHC,IF
Species ReactivityHuman,Mouse,Rat
SpecificityThe antibody detects endogenous level of total DDB1 protein.
Immunogen TypePeptide
Immunogen DescA synthetic peptide of human DDB1.
Target NameDDB1
ConjugateUnconjugated
Other NamesXPE;DDBA;XAP1;XPCE;XPE-BF;UV-DDB1;
Accession NoSwiss-Prot#: Q16531
NCBI Gene ID: 1642
Uniprot
Q16531
Gene ID
1642;
Sdspage MW127kd
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
WB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using DDB1 at 1:500 dilution.
Immunohistochemistry of paraffin-embedded rat testis using DDB1 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using DDB1 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using DDB1 at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using DDB1 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using DDB1 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
The protein encoded by this gene is the large subunit (p127) of the heterodimeric DNA damage-binding (DDB) complex while another protein (p48) forms the small subunit. This protein complex functions in nucleotide-excision repair and binds to DNA following UV damage. Defective activity of this complex causes the repair defect in patients with xeroderma pigmentosum complementation group E (XPE) - an autosomal recessive disorder characterized by photosensitivity and early onset of carcinomas. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. The protein encoded by this gene also functions as an adaptor molecule for the cullin 4 (CUL4) ubiquitin E3 ligase complex by facilitating the binding of substrates to this complex and the ubiquitination of proteins.
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et al,AMBRA1 drives gastric cancer progression through regulation of tumor plasticity
, (2024),
PMID:
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