Product Detail
Product NameGSK3B antibody
Host SpeciesRabbit
ClonalityPolyclonal
IsotypeIgG
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ApplicationsIF WB IHC IP
Species ReactivityHuman Mouse Rat
SpecificityThe antibody detects endogenous level of total GSK3B protein.
Immunogen TypePeptide
Immunogen DescThe antiserum was produced against synthesized peptide derived from human GSK3B.
Target NameGSK3B
ConjugateUnconjugated
Other NamesGSK3B;gsk-3��
Accession NoUniprot:P49841
GeneID:2932
Uniprot
P49841
Gene ID
2932
Sdspage MW46kDa
Concentration1.0mg/ml
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
StorageStore at -20˚C. Avoid freeze / thaw cycles.
Application Details
IF 1:50-200
WB 1:500-1:2000
IHC 1:100-1:300
Western blot analysis of lysates from 293, COLO205, and HT29 cells, using GSK3 beta Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from 1) 3T3, 2) HT29, 3) 293 cells, (Green) primary antibody was diluted at 1:1000 (Red) GAPDH antibody was diluted at 1:5000
Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,GSK3β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,GSK3β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,GSK3β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of rat-kidney tissue. 1,GSK3β Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
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et al,Multifunctional Injectable Bioadhesive with Toll-like Receptor 4 and Myeloid Differentiation Factor 2 Antagonistic Anti-inflammatory Potential for Periodontal Regeneration.
, (2025),
PMID:
39951685