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Bcl-2 Monoclonal Antibody#40415

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Product NameBcl-2 Monoclonal Antibody

Clone No.6B5

Host SpeciesMouse

ClonalityMonoclonal

PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

ApplicationsWB IHC IF/ICC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous Bcl-2 proteins.

Immunogen DescSynthetic peptide of human Bcl-2

Target NameBcl-2

ConjugateUnconjugated

Other NamesBCL2; Apoptosis regulator Bcl-2

Accession NoSwiss-Prot: P10415
NCBI Gene ID: 596

Uniprot P10415

Gene ID 596;

Sdspage MW26kd

Concentration0.73mg/mL

FormulationPBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

StorageStore at -20˚C/1 year

Application Details

Western blotting: 1:1000~2000
Immunohistochemistry: 1:200

IF: 1:50-200

Western blot analysis of lysates from 1)Hela cell , 2)Hela cells knockdown by siRNA1
Western blot analysis of lysates from 1)Hela, 2) MCF-7 cells, (Green) primary antibody was diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded Human breast cancer.
Immunohistochemical analysis of paraffin-embedded Human Fallopian tube.
Immunofluorescence analysis of Hela cell. 1,Dnmt3b Polyclonal Antibody(red) was diluted at 1:200(4° overnight). Bcl-2 Monoclonal Antibody(6B5)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).

If you have published an article using product 40415, please notify us so that we can cite your literature.

et al,Physalin B reduces tau phosphorylation and cell apoptosis in HEK293 cells by activating FoxO1. In Curr Mol Pharmacol on 2024 by Wei Zhang, Yating Shi,et al..PMID:37489791 , (2024),
PMID: 37489791
et al,IDH1 Overexpression Induced Chemotherapy Resistance and IDH1 Mutation Enhanced Chemotherapy Sensitivity in Glioma Cells in Vitro and in Vivo , (2014),
PMID:

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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