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p16 Polyclonal Antibody#41296

Availability: Yes
Total Amount:$
Product Detail

Product Namep16 Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB ELISA

Species ReactivityHu

Specificityp16 Polyclonal Antibody detects endogenous levels of p16 protein.

Immunogen Typepeptide

Immunogen DescSynthesized peptide derived from the N-terminal region of human p16.

Target Namep16

ConjugateUnconjugated

Other NamesCDKN2A; CDKN2; MTS1; Cyclin-dependent kinase inhibitor 2A; isoforms 1/2/3; Cyclin-dependent kinase 4 inhibitor A; CDK4I; Multiple tumor suppressor 1; MTS-1; p16-INK4a; p16-INK4; p16INK4A

Accession NoSwiss-Prot: P42771
NCBI Gene ID: 1029

Uniprot P42771

Gene ID 1029;

Sdspage MW16kd

Concentration1mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20˚C/1 year

Application Details
Western Blot: 1/500 - 1/2000.
ELISA: 1/20000.
Not yet tested in other applications.
All lanes : p16 Polyclonal Antibody at 1/500 dilution
Lane 1 : 293T whole cell lysates
Lane 2 : 293T treated with serum for 30min whole cell lysates
Lane 3 : Hela whole cell lysates
Lane 4 : Hela treated with serum for 30min whole cell lysates Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
Exposure time: 3 seconds

If you have published an article using product 41296, please notify us so that we can cite your literature.

et al,Baicalin induces cellular senescence in human colon cancer cells via upregulation of DEPP and the activation of Ras/Raf/MEK/ERK signaling.Incell Death Dis. On 2018 Feb 13 by Wang Z, Ma L et al..PMID:29440765 , (2018),
PMID: 29440765
et al,OR2H2 Activates CAMKKβ–AMPK–Autophagy Signaling Axis and Suppresses Senescence in VK2/E6E7 CellsInPharmaceuticals (Basel)On2023 SepbyJi Min Kim, Sina Dziobaka et al..PMID:37765029 , (2023),
PMID: 37765029

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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