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p27 Polyclonal Antibody#41299

Availability: Yes
Total Amount:$
Product Detail

Product Namep27 Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB IHC ELISA

Species ReactivityHu Ms Rt

Specificityp27 Polyclonal Antibody detects endogenous levels of p27 protein.

Immunogen Typepeptide

Immunogen DescSynthesized peptide derived from human p27 around the non-phosphorylation site of T187.

Target Namep27

ConjugateUnconjugated

Other NamesCDKN1B; KIP1; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1

Accession NoSwiss-Prot: P46527
NCBI Gene ID: 1027

Uniprot P46527

Gene ID 1027;

Sdspage MW26kd

Concentration1mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20˚C/1 year

Application Details
Western Blot: 1/500 - 1/2000.
Immunohistochemistry: 1/100 - 1/300.
ELISA: 1/20000.
Not yet tested in other applications.
Western Blot analysis of 293 cells using p27 Polyclonal Antibody
Western Blot analysis of COLO 293T HELA cells using p27 Polyclonal Antibody

If you have published an article using product 41299, please notify us so that we can cite your literature.

et al,Vinpocetine Inhibits Breast Cancer Cells Growth in Vitro and in Vivo.In Apoptosis on 2012 Oct by Er-Wen Huang, Sheng-Jiang Xue, et al..PMID:22729609 , (2012),
PMID: 22729609
et al,MK-2206 induces cell cycle arrest and apoptosis in HepG2 cells and sensitizes TRAIL-mediated cell death.In Mol Cell Biochem on 2013 Oct by Peng Jiao, Yun-Sheng Zhou,et al..PMID:23797319 , (2013),
PMID: 23797319
et al,Anticancer effects of sodium butyrate on hepatocellular carcinoma cells in vitro. In Int J Mol Med on 2013 Apr by Hong-Gang Wang, Xiao-Dan Huang, et al..PMID: 23440283 , (2013),
PMID: 23440283
et al,Cloning and characterization of a novel intracellular protein p48. 2 that negatively regulates cell cycle progression. In Int J Biochem Cell Biol on 2009 Nov by Fan Yang, Yu-Ping Xu, et al..PMID:19427400 , (2009),
PMID: 19427400
et al,Blocking the bFGF/STAT3 interaction through specific signaling pathways induces apoptosis in glioblastoma cells.In J Neurooncol 2014 Oct by Jingchao Wu , Xuequan Feng et al..PMID: 25048528 , (2014),
PMID: 25048528
et al,EEN regulates the proliferation and survival of multiple myeloma cells by potentiating IGF-1 secretion.In Biochem Biophys Res Commun on 2014 May 2 by Er-Wen Huang , Sheng-Jiang Xue et al ..PMID: 24704450 , (2014),
PMID: 24704450
et al,Insights in dynamic kinome reprogramming as a consequence of MEK inhibition in MLL-rearranged AML.In Leukemia on 2014 Mar by K R Kampen, A Ter Elst et al..PMID:24240200 , (2014),
PMID: 24240200
More
et al,Elucidating the role of the FoxO3a transcription factor in the IGF-1-induced migration and invasion of uveal melanoma cancer cells.In Biomed Pharmacother on 2016 Dec by Fengxia Yan , Rifang Liao et al..PMID: 27881235 , (2016),
PMID: 27881235
et al,Screening active components from Rubus amabilis for pancreatic β-cells protection. In Pharm Biol on 2020 Dec by Min Sun, Tiantian Zhu, et al..PMID: 32659127 , (2020),
PMID: 32659127
et al,AMBRA1 drives gastric cancer progression through regulation of tumor plasticity , (2024),
PMID:

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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