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PDK2 Polyclonal Antibody#41330

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Product NamePDK2 Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB IHC ELISA

Species ReactivityHu Ms Rt

SpecificityPDK2 Polyclonal Antibody detects endogenous levels of PDK2 protein.

Immunogen DescSynthesized peptide derived from the Internal region of human PDK2.

Target NamePDK2

ConjugateUnconjugated

Other NamesPDK2; PDHK2; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2; mitochondrial; Pyruvate dehydrogenase kinase isoform 2

Accession NoSwiss-Prot: Q15119
NCBI Gene ID: 5164

Uniprot Q15119

Gene ID 5164;

Sdspage MW46kd

Concentration1mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20˚C/1 year

Application Details

WB 1:500 - 1:2000 IHC 1:100 - 1:300 ELISA: 1:20000 IF 1:50-200

Western Blot analysis of HepG2 cells using PDK2 Polyclonal Antibody
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

If you have published an article using product 41330, please notify us so that we can cite your literature.

et al,Huzhangoside A Suppresses Tumor Growth through Inhibition of Pyruvate Dehydrogenase KInase Activity.In Cancers (Basel). On 2019 May 23 by Kwak CH, Lee JH, et al.. PMID: 31126094 , (2019),
PMID: 31126094
et al,Ilimaquinone Induces the Apoptotic Cell Death of Cancer Cells by Reducing Pyruvate Dehydrogenase Kinase 1 Activity. In Int J Mol Sci on 2020 Aug 21 by Choong-Hwan Kwak, Ling Jin, et al..PMID:32825675 , (2020),
PMID: 32825675
et al,Targeting Lactate Dehydrogenase A with Catechin Resensitizes SNU620/5FU Gastric Cancer Cells to 5-Fluorouracil. In Int J Mol Sci on 2021 May 20 by Jung Ho Han, MinJeong Kim,et al..PMID:34065602 , (2021),
PMID: 34065602

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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