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Siah-1/2 Polyclonal Antibody#41434

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Total Amount:$
Product Detail

Product NameSiah-1/2 Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB IHC IF ELISA

Species ReactivityHu Ms Rt

SpecificitySiah-1/2 Polyclonal Antibody detects endogenous levels of Siah-1/2 protein.

Immunogen Typepeptide

Immunogen DescSynthesized peptide derived from the Internal region of human Siah-1/2.

Target NameSiah-1/2

ConjugateUnconjugated

Other NamesSIAH1; HUMSIAH; E3 ubiquitin-protein ligase SIAH1; Seven in absentia homolog 1; Siah-1; Siah-1a; SIAH2; E3 ubiquitin-protein ligase SIAH2; Seven in absentia homolog 2; Siah-2; hSiah2

Accession NoSwiss-Prot: Q8IUQ4/O43255
NCBI Gene ID: 6477/6478

Uniprot Q8IUQ4

Gene ID 6477;

Sdspage MW34kd

Concentration1mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20˚C/1 year

Application Details
Western Blot: 1/500 - 1/2000.
Immunohistochemistry: 1/100 - 1/300.
Immunofluorescence: 1/200 - 1/1000.
ELISA: 1/10000.
Not yet tested in other applications.
Western Blot analysis of HeLa cells using Siah-1/2 Polyclonal Antibody
Western Blot analysis of RAT-MUSCLE cells using Siah-1/2 Polyclonal Antibody

If you have published an article using product 41434, please notify us so that we can cite your literature.

et al,MYPT1 Downregulation by LipopolysaccharideSIAH1/2 E3 LigaseUbiquitin-Proteasomal Degradation Contributes to Colonic Obstruction of Hirschsprung Disease.In Cell Mol Gastroenterol Hepatol on 2020 by Zhao W, Wang P et al..PMID:31759145 , (2020),
PMID: 31759145

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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