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Location： Home > Polyclonal Antibodies > TNF-α Polyclonal Antibody

TNF-α Polyclonal Antibody#41504

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Product Detail

Product NameTNF-α Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB IHC IF ELISA

Species ReactivityHu Ms Rt

SpecificityTNF-α Polyclonal Antibody detects endogenous levels of TNF-α protein.

Immunogen Typepeptide

Immunogen DescSynthesized peptide derived from the Internal region of human TNF-α

Target NameTNF-α

ConjugateUnconjugated

Other NamesTNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a

Accession NoSwiss-Prot: P01375
NCBI Gene ID: 7124

Uniprot P01375

Gene ID 7124;

Sdspage MW16kd

Concentration1mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20˚C/1 year

Application Details
Western Blot: 1:500-2000
Immunohistochemistry: 1/100 - 1/300
Immunofluorescence: 1/200 - 1/1000
ELISA: 1/10000
Western Blot analysis of Jurkat cells using TNF-α Polyclonal Antibody
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using TNFA antibody.

If you have published an article using product 41504, please notify us so that we can cite your literature.

et al,Two-way immune effects of deoxynivalenol in weaned piglets and porcine alveolar macrophages: Due mainly to its exposure dosage.In Chemosphere on 2020 Jun; by Liu D, Wang Q, et al..PMID:32229367 , (2020),
PMID: 32229367
et al,Tongxinluo inhibits vascular inflammation and neointimal hyperplasia through blockade of the positive feedback loop between miR-155 and TNF-伪.In Am J Physiol Heart Circ Physiol on 2014 Aug 15 et al..PMID: 24951754 , (2014),
PMID: 24951754
et al,Bifico Relieves Irritable Bowel Syndrome By Regulating Gut Microbiota Dysbiosis and Inflammatory Cytokines in an Animal Mode , (2021),
PMID:
et al,Bifico relieves irritable bowel syndrome by regulating gut microbiota dysbiosis and inflammatory cytokines. In Eur J Nutr on 2023 Feb by Yanlin Zhou, Fan Zhang,et al..PMID:35918555 , (2022),
PMID: 35918555
et al,Reproductive endocrine disruption and gonadal intersex induction in male Japanese medaka chronically exposed to betamethasone at environmentally relevant levelsInJ Hazard MaterOn2023 Aug 5byMaoliang Su , Youling Zhong et al..PMID:37156043 , (2023),
PMID: 37576825
et al,Bifico relieves irritable bowel syndrome by regulating gut microbiota dysbiosis and inflammatory cytokinesIn Eur J Nutr On2023 FebbyYanlin Zhou , Fan Zhang et al..PMID:35918555 , (2023),
PMID: 35918555
et al,Coffea arabica Extract Attenuates Atopic Dermatitis-like Skin Lesions by Regulating NLRP3 Inflammasome Expression and Skin Barrier FunctionsIn Int J Mol SciOn2023 Aug 2byQiao-Xin Chang , Jia-Ling Lyu et al..PMID:37569742 , (2023),
PMID: 37569742
More
et al,Berberine inhibits high fat diet-associated colorectal cancer through modulation of the gut microbiota-mediated lysophosphatidylcholine InInt J Biol SciOn 2023 Apr 9byHaitao Chen , Chenxiao Ye et al..PMID:37151876 , (2023),
PMID: 37151876
et al,Qing Hua Chang Yin alleviates chronic colitis of mice by protecting intestinal barrier function and improving colonic microflora , (2023),
PMID: 37576825
et al,Lipopolysaccharide aggravating anaphylactoid reactions caused by traditional Chinese Medicine injections via p38/ERK/NF-κB signaling pathways. , (2025),
PMID: 39419299

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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