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α-SMA Polyclonal Antibody#41550

Availability: Yes
Total Amount:$
Product Detail

Product Nameα-SMA Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB IHC ELISA IF

Species ReactivityHu Ms Rt

Specificityα-SMA Polyclonal Antibody detects endogenous levels of α-SMA protein.

Immunogen DescSynthesized peptide derived from the N-terminal region of human α-SMA.

Target Nameα-SMA

ConjugateUnconjugated

Other NamesACTA2; ACTSA; ACTVS; GIG46; Actin; aortic smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 protein

Accession NoSwiss-Prot: P62736
NCBI Gene ID: 59

Uniprot P62736

Gene ID 59;

Sdspage MW42kd

Concentration1mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20˚C/1 year

Application Details

Western Blot: 1/500 - 1/2000.
Immunohistochemistry: 1/100 - 1/300.
ELISA: 1/20000.
Not yet tested in other applications.

Western Blot analysis of Jurkat K562 HuvEc cells using α-SMA Polyclonal Antibody

If you have published an article using product 41550, please notify us so that we can cite your literature.

et al,TGF β1 mediated Smad signaling pathway and EMT in hepatic fibrosis induced by Nano NiO in vivo and in vitro.In Environ Toxicol on 2020 Apr by Zhang Q, Chang X ,et al..PMID: 31737983 , (2020),
PMID: 31737983
et al,Everolimus Alleviates Renal Allograft Interstitial Fibrosis by Inhibiting Epithelial-to-Mesenchymal Transition Not Only via Inducing Autophagy but Also via Stabilizing IκB-α.In Front Immunol. 2022 Jan 24 by Zeping Gui , Chuanjian Suo,et al.PMID: 35140705 , (2022),
PMID: 35140705
et al,Telmisartan inhibits bladder smooth muscle fibrosis in neurogenic bladder rats. In Exp Ther Med on 2022 Mar by Qian Liu, Ruoyi Wang, et al..PMID:35126719 , (2022),
PMID: 35126719

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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