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Location： Home > Mouse Monoclonal Antibodies > ENO2/NSE Monoclonal Antibody

ENO2/NSE Monoclonal Antibody#42020

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Product Detail

Product NameENO2/NSE Monoclonal Antibody

Host SpeciesMouse

ClonalityMonoclonal

Purificationprotein G purifed

ApplicationsWB IHC

Species ReactivityHu

Specificityspecific for Human ENO2 denatured and native forms

Immunogen Typeprotein

Immunogen DescRecombinant Human ENO2

Target NameENO2/NSE

ConjugateUnconjugated

Other Names2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE, ENO2

Accession NoSwiss-Prot#: P09104

Uniprot P09104

Gene ID 2026;

Calculated MW48kd

Concentration1.0mg/mL

FormulationPreservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

StorageStore at -20˚C

Application Details
Western blotting: 1:500 - 1:1000
Immunohistochemistry: 1:20 - 1:200
All Lanes Mouse anti-human ENO2 monoclonal antibody at 1ug/ml Lane 1 Hela cell lysate Lane 2 Recombinant Human Gamma-enolase(ENO2) at 10ug Lane 3 U251 cell lysate Secondary Goat polyclonal to Mouse IgG at 1/3000 dilution Predicted band size : 48 kDa Observed band size: 48kDa Additional bands at : 100kDa. We are unsure as to the identity of this extra band.
Immunohistochemical analysis of paraffin-embedded human small intestine using #42020 at dilution of 1:200.
Immunohistochemical analysis of paraffin-embedded human pancreas using #42020 at dilution of 1:200.
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival

If you have published an article using product 42020, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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