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Caspase-3 Polyclonal Antibody#42437

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Product NameCaspase-3 Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationCaprylic Acid Ammonium Sulfate Precipitation purified

ApplicationsWB IHC

Species ReactivityHu Ms

SpecificityThe antibody detects endogenous level of total Caspase-3 polyclonal antibody.

Immunogen Typeprotein

Immunogen DescRecombinant human Caspase-3 protein

Target NameCaspase-3

ConjugateUnconjugated

Other NamesApopain, Cysteine protease CPP32Protein Yama, SREBP cleavage activity 1

Accession NoSwiss-Prot#: P42574

Uniprot P42574

Gene ID 836;

Calculated MW30kd

FormulationPreservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

StorageStore at -20˚C

Application Details
Western blotting: 1:500 - 1:1000
Immunohistochemistry: 1:20 - 1:200
All lanes: Caspase-3 antibody at 2ug/ml+mouse brain tissue secondary Goat polyclonal to rabbit at 1/10000 dilution predicted band size :30kDa observed band size :30kDa
Immunohistochemical analysis of paraffin-embedded human colon cancer using #42437 at dilution of 1:100.
Involved in the activation cascade of caspases responsible for apoptosis execution.

If you have published an article using product 42437, please notify us so that we can cite your literature.

et al,Baicalin induces cellular senescence in human colon cancer cells via upregulation of DEPP and the activation of Ras/Raf/MEK/ERK signaling.Incell Death Dis. On 2018 Feb 13 by Wang Z, Ma L et al..PMID:29440765 , (2018),
PMID: 29440765

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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