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UBE2J2 Antibody#43366

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Product Detail

Product NameUBE2J2 Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntigen affinity purification.

ApplicationsWB IHC

Species ReactivityHu Ms

SpecificityThe antibody detects endogenous levels of total UBE2J2 protein.

Immunogen DescFusion protein of human UBE2J2

Target NameUBE2J2

ConjugateUnconjugated

Other NamesNCUBE2; NCUBE-2; PRO2121

Accession NoSwiss-Prot#: Q8N2K1
Gene ID: 118424

Uniprot Q8N2K1

Gene ID 118424;

Calculated MW29kd

Concentration1.1mg/ml

FormulationRabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

StorageStore at -20˚C

Application Details
Western blotting: 1:200-1:1000
Immunohistochemistry: 1:20-1:100

 

Gel: 12%SDS-PAGE
Lysate: 40 μg
Lane 1-3: 293T cell,Mouse skeletal muscle tissue and HEPG2 cell
Primary antibody: 1/250 dilution
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 3 seconds
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #43366 at dilution 1/20,

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum.

If you have published an article using product 43366, please notify us so that we can cite your literature.

et al,A Pyroptosis-Related Gene Signature Predicts Prognosis and Tumor Immune Microenvironment in Colorectal Cancer. In Technol Cancer Res Treat on 2024 Jan-Dec by Linjing Li, Yuyi Li,et al..PMID: 39155627 , (2024),
PMID: 39155627

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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